| 1. | Agrin inhibits rac activity in developing neurons and expression of dominant negative rac1 abolishes agrin - induced growth cone turning and substantially inhibits neurite extension
我们的实验也表明， agrin抑制发育中神经元rac的活性。 dn - rac1的表达完全阻断了agrin引起的生长锥导向，并抑制突起的生长。 |
| 2. | 2 ) the growth curves for different cells show that overexpression of gpil7p has no lethal effect on the yeast cells and only that of the dominant negative mutants can cause cells not grow
2 )生长曲线的测定表明，过表达gpi17p对正常酵母细胞不致死，而只有显性负性突变体的过表达才会导致酵母细胞不能生长。 |
| 3. | Interestingly , inhibition of p38 mapk activation ( but not erk activation ) by its inhibitors or by expression of a dominant negative mutant of p38 mapk could effectively block the chemotactic effect of ( - arrestin2
P38mapk上游激酶ask1的显性失活突变体也可以阻断- arrestin2对趋化的增强作用。这说明是p38mapk而不是erk信号通路与- arrestin2对趋化的增强作用相关。 |
| 4. | Expression of either dominant negative or antisense musk in cultured xenopus laveis spinal neurons abolishes agrin - induced neurite outgrowth inhibition and converts chemorepulsive growth cone turning induced by a gradient of agrin into chemoattraction
Musk突变体（ dm - musk ）或反义musk ( as - musk )的表达，解除了agrin对新生突起生长的抑制，并且把agrin诱导的排斥反应反转为吸引反应； musk介导发育中神经元突起的生长。 |
| 5. | 3 ) foa ( fluoroorotic acid ) treatment with the dominant negative mutants showed that the dominant negative effects of mutants were caused by the presence of the gpi17 vector , not by another mutation located somewhere in the genome and in a gene involved in the natural resistance against copper
3 ) foa ( 5 -氟-乳清酸)处理突变体细胞表明，得到的显性负性突变体不是细胞中铜抗性基因突变的产物，其确为gpi17突变引起。 |
| 6. | Dominant negative alleles of gpil7p were produced by random pcr mutagenesis of three conserved fragments of gpi17p : 1 ) having generated the plasmid library which contains 100000 plasmids harboring gpi17 mutants then screened from 8000 clones , we got 25 strong and 3 very strong dominant negative alleles
对gpi17p可能的三个保守区进行了pcr随机诱变，并最终获得显性负性等位基因： 1 )组建了gpi17p突变体质粒库，其所含质粒数约为100000 。 |
